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4. Condition Supelclean™ Envi™ Carb SPE cartridge with 6 mL acetonitrile, adopted by 6 mL 1% acetic acid in water. When decanting supernatant into SPE, some particulates could enter SPE. After supernatant has utterly passed through, wash SPE with 6 mL of deionized water and acquire eluant within the above collection vessel. 6. Adjust last quantity of pattern extract to forty mL with deionized water and briefly vortex centrifuge tube.

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